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vitro nrf2 knockdown  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology vitro nrf2 knockdown
    Sfn reduces neuronal death in vivo and in vitro. Six weeks after 2VO, brain sections at dorsal hippocampus were collected and stained with LFB-CV. (a) Representative images of the parietal cortex (upper panels, scale bar = 100 µm) and hippocampal CA1 (lower panels, scale bar = 200 µm). (b, c) Neurons with normal-like morphology were counted and data were expressed as number per mm2. 2VO reduced normal-like neurons in both cortex and CA1, which was partially reversed by Sfn. n = 6, *p < 0.05, **p < 0.01, ***p < 0.001 vs. Sham; ##p < 0.01, ###p < 0.001 vs. 2VO. <t>Nrf2</t> knockdown in rat primary neurons was induced by sh-Nrf2 transfection, and OGD was performed with or without Sfn pretreatment. (d) Hoechst 33258 staining with cell counting and (e) LDH assay showed that Sfn ameliorated OGD-induced cell death, which was abolished by Nrf2 knockdown. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Ctrl; #p < 0.05, ##p < 0.01 vs. OGD; &p<0.05 vs. OGD+Sfn.
    Vitro Nrf2 Knockdown, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vitro nrf2 knockdown/product/Santa Cruz Biotechnology
    Average 93 stars, based on 24 article reviews
    vitro nrf2 knockdown - by Bioz Stars, 2026-02
    93/100 stars

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    1) Product Images from "Protective effects of sulforaphane in experimental vascular cognitive impairment: Contribution of the Nrf2 pathway"

    Article Title: Protective effects of sulforaphane in experimental vascular cognitive impairment: Contribution of the Nrf2 pathway

    Journal: Journal of Cerebral Blood Flow & Metabolism

    doi: 10.1177/0271678X18764083

    Sfn reduces neuronal death in vivo and in vitro. Six weeks after 2VO, brain sections at dorsal hippocampus were collected and stained with LFB-CV. (a) Representative images of the parietal cortex (upper panels, scale bar = 100 µm) and hippocampal CA1 (lower panels, scale bar = 200 µm). (b, c) Neurons with normal-like morphology were counted and data were expressed as number per mm2. 2VO reduced normal-like neurons in both cortex and CA1, which was partially reversed by Sfn. n = 6, *p < 0.05, **p < 0.01, ***p < 0.001 vs. Sham; ##p < 0.01, ###p < 0.001 vs. 2VO. Nrf2 knockdown in rat primary neurons was induced by sh-Nrf2 transfection, and OGD was performed with or without Sfn pretreatment. (d) Hoechst 33258 staining with cell counting and (e) LDH assay showed that Sfn ameliorated OGD-induced cell death, which was abolished by Nrf2 knockdown. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Ctrl; #p < 0.05, ##p < 0.01 vs. OGD; &p<0.05 vs. OGD+Sfn.
    Figure Legend Snippet: Sfn reduces neuronal death in vivo and in vitro. Six weeks after 2VO, brain sections at dorsal hippocampus were collected and stained with LFB-CV. (a) Representative images of the parietal cortex (upper panels, scale bar = 100 µm) and hippocampal CA1 (lower panels, scale bar = 200 µm). (b, c) Neurons with normal-like morphology were counted and data were expressed as number per mm2. 2VO reduced normal-like neurons in both cortex and CA1, which was partially reversed by Sfn. n = 6, *p < 0.05, **p < 0.01, ***p < 0.001 vs. Sham; ##p < 0.01, ###p < 0.001 vs. 2VO. Nrf2 knockdown in rat primary neurons was induced by sh-Nrf2 transfection, and OGD was performed with or without Sfn pretreatment. (d) Hoechst 33258 staining with cell counting and (e) LDH assay showed that Sfn ameliorated OGD-induced cell death, which was abolished by Nrf2 knockdown. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Ctrl; #p < 0.05, ##p < 0.01 vs. OGD; &p<0.05 vs. OGD+Sfn.

    Techniques Used: In Vivo, In Vitro, Staining, Knockdown, Transfection, Cell Counting, Lactate Dehydrogenase Assay

    Sfn protects the BBB via Nrf2 activation and TJ preservation. MBMECs were subjected to OGD with or without Sfn pretreatment. (a) Live/dead staining (scale bar = 50 µm) with cell counting and (b) LDH assay showed that Sfn protected against OGD-induced cell death. (c) In an in vitro BBB model, Sfn partially reversed OGD-induced BBB leakage. ***p < 0.001 vs. Ctrl; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. OGD. Nrf2 was knocked down by sh-Nrf2 transfection. (d) Live/dead staining (scale bar = 50 µm) with cell counting and (e) LDH assay revealed that Sfn-provided protection was abolished by Nrf2 knockdown. n.s.: not significant, **p < 0.01. (f) In vitro BBB permeability assessment without OGD showed that Nrf2 knockdown disrupted the BBB integrity. *p < 0.05, **p < 0.01, ***p < 0.001 vs. sh-Sc. (g) Dual-luciferase assay revealed that CLD5 promoter activity was decreased by Nrf2 knockdown. n.s.: not significant, **p < 0.01, ***p < 0.001. (h) Six weeks after 2VO, the brain sections were collected and immunohistochemistry showed that Sfn reduced CLD5 loss in the cortex (scale bar = 20 µm). (i–k) Representative Western blots and semi-quantitative analysis showed that Sfn reduced loss of CLD5 and occludin after 2VO. *p < 0.05 vs. Sham; #p < 0.05, ##p < 0.01 vs. 2VO.
    Figure Legend Snippet: Sfn protects the BBB via Nrf2 activation and TJ preservation. MBMECs were subjected to OGD with or without Sfn pretreatment. (a) Live/dead staining (scale bar = 50 µm) with cell counting and (b) LDH assay showed that Sfn protected against OGD-induced cell death. (c) In an in vitro BBB model, Sfn partially reversed OGD-induced BBB leakage. ***p < 0.001 vs. Ctrl; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. OGD. Nrf2 was knocked down by sh-Nrf2 transfection. (d) Live/dead staining (scale bar = 50 µm) with cell counting and (e) LDH assay revealed that Sfn-provided protection was abolished by Nrf2 knockdown. n.s.: not significant, **p < 0.01. (f) In vitro BBB permeability assessment without OGD showed that Nrf2 knockdown disrupted the BBB integrity. *p < 0.05, **p < 0.01, ***p < 0.001 vs. sh-Sc. (g) Dual-luciferase assay revealed that CLD5 promoter activity was decreased by Nrf2 knockdown. n.s.: not significant, **p < 0.01, ***p < 0.001. (h) Six weeks after 2VO, the brain sections were collected and immunohistochemistry showed that Sfn reduced CLD5 loss in the cortex (scale bar = 20 µm). (i–k) Representative Western blots and semi-quantitative analysis showed that Sfn reduced loss of CLD5 and occludin after 2VO. *p < 0.05 vs. Sham; #p < 0.05, ##p < 0.01 vs. 2VO.

    Techniques Used: Activation Assay, Preserving, Staining, Cell Counting, Lactate Dehydrogenase Assay, In Vitro, Transfection, Knockdown, Permeability, Luciferase, Activity Assay, Immunohistochemistry, Western Blot

    Sfn activates Nrf2 and upregulates HO-1 expression after 2VO in rats. Cortical tissues were harvested at indicated time-points, and nuclear and cytosolic proteins were extracted. (a) Representative Western blots and semi-quantitative analysis within one week after 2VO, showing that 2VO-induced Nrf2 activation was enhanced by Sfn administration. (b) Representative Western blots and semi-quantitative at six weeks after 2VO, showing long-lasting activation of Nrf2 pathway by Sfn. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Sham; #p < 0.05, ##p < 0.01 vs. 2VO. (c) Representative images of double-labeling of HO-1 with cellular markers (NeuN for neurons, GFAP for astrocytes and microvessels, Iba-1 for microglia/macrophages, and APC for mature oligodendrocytes) revealed wide-spread HO-1 expression after 2VO. Scale bar = 50 µm.
    Figure Legend Snippet: Sfn activates Nrf2 and upregulates HO-1 expression after 2VO in rats. Cortical tissues were harvested at indicated time-points, and nuclear and cytosolic proteins were extracted. (a) Representative Western blots and semi-quantitative analysis within one week after 2VO, showing that 2VO-induced Nrf2 activation was enhanced by Sfn administration. (b) Representative Western blots and semi-quantitative at six weeks after 2VO, showing long-lasting activation of Nrf2 pathway by Sfn. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Sham; #p < 0.05, ##p < 0.01 vs. 2VO. (c) Representative images of double-labeling of HO-1 with cellular markers (NeuN for neurons, GFAP for astrocytes and microvessels, Iba-1 for microglia/macrophages, and APC for mature oligodendrocytes) revealed wide-spread HO-1 expression after 2VO. Scale bar = 50 µm.

    Techniques Used: Expressing, Western Blot, Activation Assay, Labeling



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    Santa Cruz Biotechnology vitro nrf2 knockdown
    Sfn reduces neuronal death in vivo and in vitro. Six weeks after 2VO, brain sections at dorsal hippocampus were collected and stained with LFB-CV. (a) Representative images of the parietal cortex (upper panels, scale bar = 100 µm) and hippocampal CA1 (lower panels, scale bar = 200 µm). (b, c) Neurons with normal-like morphology were counted and data were expressed as number per mm2. 2VO reduced normal-like neurons in both cortex and CA1, which was partially reversed by Sfn. n = 6, *p < 0.05, **p < 0.01, ***p < 0.001 vs. Sham; ##p < 0.01, ###p < 0.001 vs. 2VO. <t>Nrf2</t> knockdown in rat primary neurons was induced by sh-Nrf2 transfection, and OGD was performed with or without Sfn pretreatment. (d) Hoechst 33258 staining with cell counting and (e) LDH assay showed that Sfn ameliorated OGD-induced cell death, which was abolished by Nrf2 knockdown. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Ctrl; #p < 0.05, ##p < 0.01 vs. OGD; &p<0.05 vs. OGD+Sfn.
    Vitro Nrf2 Knockdown, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vitro nrf2 knockdown/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    vitro nrf2 knockdown - by Bioz Stars, 2026-02
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    Sfn reduces neuronal death in vivo and in vitro. Six weeks after 2VO, brain sections at dorsal hippocampus were collected and stained with LFB-CV. (a) Representative images of the parietal cortex (upper panels, scale bar = 100 µm) and hippocampal CA1 (lower panels, scale bar = 200 µm). (b, c) Neurons with normal-like morphology were counted and data were expressed as number per mm2. 2VO reduced normal-like neurons in both cortex and CA1, which was partially reversed by Sfn. n = 6, *p < 0.05, **p < 0.01, ***p < 0.001 vs. Sham; ##p < 0.01, ###p < 0.001 vs. 2VO. Nrf2 knockdown in rat primary neurons was induced by sh-Nrf2 transfection, and OGD was performed with or without Sfn pretreatment. (d) Hoechst 33258 staining with cell counting and (e) LDH assay showed that Sfn ameliorated OGD-induced cell death, which was abolished by Nrf2 knockdown. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Ctrl; #p < 0.05, ##p < 0.01 vs. OGD; &p<0.05 vs. OGD+Sfn.

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Protective effects of sulforaphane in experimental vascular cognitive impairment: Contribution of the Nrf2 pathway

    doi: 10.1177/0271678X18764083

    Figure Lengend Snippet: Sfn reduces neuronal death in vivo and in vitro. Six weeks after 2VO, brain sections at dorsal hippocampus were collected and stained with LFB-CV. (a) Representative images of the parietal cortex (upper panels, scale bar = 100 µm) and hippocampal CA1 (lower panels, scale bar = 200 µm). (b, c) Neurons with normal-like morphology were counted and data were expressed as number per mm2. 2VO reduced normal-like neurons in both cortex and CA1, which was partially reversed by Sfn. n = 6, *p < 0.05, **p < 0.01, ***p < 0.001 vs. Sham; ##p < 0.01, ###p < 0.001 vs. 2VO. Nrf2 knockdown in rat primary neurons was induced by sh-Nrf2 transfection, and OGD was performed with or without Sfn pretreatment. (d) Hoechst 33258 staining with cell counting and (e) LDH assay showed that Sfn ameliorated OGD-induced cell death, which was abolished by Nrf2 knockdown. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Ctrl; #p < 0.05, ##p < 0.01 vs. OGD; &p<0.05 vs. OGD+Sfn.

    Article Snippet: In vitro Nrf2 knockdown To knock down Nrf2 in neurons and MBMECs, lentiviral particles carrying shRNA targeting Nrf2 (sh-Nrf2) or a scramble sequence (sh-Sc) were added to cells together with 8 μg/ml polybrene (all from Santa Cruz, Dallas, TX) for 16 h. The virus-containing culture media were then replaced with fresh growth media.

    Techniques: In Vivo, In Vitro, Staining, Knockdown, Transfection, Cell Counting, Lactate Dehydrogenase Assay

    Sfn protects the BBB via Nrf2 activation and TJ preservation. MBMECs were subjected to OGD with or without Sfn pretreatment. (a) Live/dead staining (scale bar = 50 µm) with cell counting and (b) LDH assay showed that Sfn protected against OGD-induced cell death. (c) In an in vitro BBB model, Sfn partially reversed OGD-induced BBB leakage. ***p < 0.001 vs. Ctrl; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. OGD. Nrf2 was knocked down by sh-Nrf2 transfection. (d) Live/dead staining (scale bar = 50 µm) with cell counting and (e) LDH assay revealed that Sfn-provided protection was abolished by Nrf2 knockdown. n.s.: not significant, **p < 0.01. (f) In vitro BBB permeability assessment without OGD showed that Nrf2 knockdown disrupted the BBB integrity. *p < 0.05, **p < 0.01, ***p < 0.001 vs. sh-Sc. (g) Dual-luciferase assay revealed that CLD5 promoter activity was decreased by Nrf2 knockdown. n.s.: not significant, **p < 0.01, ***p < 0.001. (h) Six weeks after 2VO, the brain sections were collected and immunohistochemistry showed that Sfn reduced CLD5 loss in the cortex (scale bar = 20 µm). (i–k) Representative Western blots and semi-quantitative analysis showed that Sfn reduced loss of CLD5 and occludin after 2VO. *p < 0.05 vs. Sham; #p < 0.05, ##p < 0.01 vs. 2VO.

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Protective effects of sulforaphane in experimental vascular cognitive impairment: Contribution of the Nrf2 pathway

    doi: 10.1177/0271678X18764083

    Figure Lengend Snippet: Sfn protects the BBB via Nrf2 activation and TJ preservation. MBMECs were subjected to OGD with or without Sfn pretreatment. (a) Live/dead staining (scale bar = 50 µm) with cell counting and (b) LDH assay showed that Sfn protected against OGD-induced cell death. (c) In an in vitro BBB model, Sfn partially reversed OGD-induced BBB leakage. ***p < 0.001 vs. Ctrl; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. OGD. Nrf2 was knocked down by sh-Nrf2 transfection. (d) Live/dead staining (scale bar = 50 µm) with cell counting and (e) LDH assay revealed that Sfn-provided protection was abolished by Nrf2 knockdown. n.s.: not significant, **p < 0.01. (f) In vitro BBB permeability assessment without OGD showed that Nrf2 knockdown disrupted the BBB integrity. *p < 0.05, **p < 0.01, ***p < 0.001 vs. sh-Sc. (g) Dual-luciferase assay revealed that CLD5 promoter activity was decreased by Nrf2 knockdown. n.s.: not significant, **p < 0.01, ***p < 0.001. (h) Six weeks after 2VO, the brain sections were collected and immunohistochemistry showed that Sfn reduced CLD5 loss in the cortex (scale bar = 20 µm). (i–k) Representative Western blots and semi-quantitative analysis showed that Sfn reduced loss of CLD5 and occludin after 2VO. *p < 0.05 vs. Sham; #p < 0.05, ##p < 0.01 vs. 2VO.

    Article Snippet: In vitro Nrf2 knockdown To knock down Nrf2 in neurons and MBMECs, lentiviral particles carrying shRNA targeting Nrf2 (sh-Nrf2) or a scramble sequence (sh-Sc) were added to cells together with 8 μg/ml polybrene (all from Santa Cruz, Dallas, TX) for 16 h. The virus-containing culture media were then replaced with fresh growth media.

    Techniques: Activation Assay, Preserving, Staining, Cell Counting, Lactate Dehydrogenase Assay, In Vitro, Transfection, Knockdown, Permeability, Luciferase, Activity Assay, Immunohistochemistry, Western Blot

    Sfn activates Nrf2 and upregulates HO-1 expression after 2VO in rats. Cortical tissues were harvested at indicated time-points, and nuclear and cytosolic proteins were extracted. (a) Representative Western blots and semi-quantitative analysis within one week after 2VO, showing that 2VO-induced Nrf2 activation was enhanced by Sfn administration. (b) Representative Western blots and semi-quantitative at six weeks after 2VO, showing long-lasting activation of Nrf2 pathway by Sfn. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Sham; #p < 0.05, ##p < 0.01 vs. 2VO. (c) Representative images of double-labeling of HO-1 with cellular markers (NeuN for neurons, GFAP for astrocytes and microvessels, Iba-1 for microglia/macrophages, and APC for mature oligodendrocytes) revealed wide-spread HO-1 expression after 2VO. Scale bar = 50 µm.

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Protective effects of sulforaphane in experimental vascular cognitive impairment: Contribution of the Nrf2 pathway

    doi: 10.1177/0271678X18764083

    Figure Lengend Snippet: Sfn activates Nrf2 and upregulates HO-1 expression after 2VO in rats. Cortical tissues were harvested at indicated time-points, and nuclear and cytosolic proteins were extracted. (a) Representative Western blots and semi-quantitative analysis within one week after 2VO, showing that 2VO-induced Nrf2 activation was enhanced by Sfn administration. (b) Representative Western blots and semi-quantitative at six weeks after 2VO, showing long-lasting activation of Nrf2 pathway by Sfn. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Sham; #p < 0.05, ##p < 0.01 vs. 2VO. (c) Representative images of double-labeling of HO-1 with cellular markers (NeuN for neurons, GFAP for astrocytes and microvessels, Iba-1 for microglia/macrophages, and APC for mature oligodendrocytes) revealed wide-spread HO-1 expression after 2VO. Scale bar = 50 µm.

    Article Snippet: In vitro Nrf2 knockdown To knock down Nrf2 in neurons and MBMECs, lentiviral particles carrying shRNA targeting Nrf2 (sh-Nrf2) or a scramble sequence (sh-Sc) were added to cells together with 8 μg/ml polybrene (all from Santa Cruz, Dallas, TX) for 16 h. The virus-containing culture media were then replaced with fresh growth media.

    Techniques: Expressing, Western Blot, Activation Assay, Labeling